Heavy-atom derivatization
نویسندگان
چکیده
منابع مشابه
Heavy-atom derivatization.
Most of the standard methods of solving macromolecular structures involve producing a protein crystal that is derivatized by an anomalous scatterer or heavy atom (MIR, SIRAS, MAD, SAD etc.). The theoretical methodology which underpins the extraction of phase information from such derivatives is widely available in the literature. In addition, there are comprehensive sources of information on th...
متن کاملAn overview of heavy-atom derivatization of protein crystals.
Heavy-atom derivatization is one of the oldest techniques for obtaining phase information for protein crystals and, although it is no longer the first choice, it remains a useful technique for obtaining phases for unknown structures and for low-resolution data sets. It is also valuable for confirming the chain trace in low-resolution electron-density maps. This overview provides a summary of th...
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A pair of isostructural bis-selenathiazolyl and bis-diselenazolyl radical conductors display weak (spin-canted) ferromagnetism with Tc values of 18 K and 27 K respectively.
متن کاملANODE: anomalous and heavy-atom density calculation
The new program ANODE estimates anomalous or heavy-atom density by reversing the usual procedure for experimental phase determination by methods such as single- and multiple-wavelength anomalous diffraction and single isomorphous replacement anomalous scattering. Instead of adding a phase shift to the heavy-atom phases to obtain a starting value for the native protein phase, this phase shift is...
متن کاملA rational approach to heavy-atom derivative screening
Despite the development in recent times of a range of techniques for phasing macromolecules, the conventional heavy-atom derivatization method still plays a significant role in protein structure determination. However, this method has become less popular in modern high-throughput oriented crystallography, mostly owing to its trial-and-error nature, which often results in lengthy empirical searc...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography
سال: 2003
ISSN: 0907-4449
DOI: 10.1107/s0907444903012794